World Scientific
  • Search
  •   
Skip main navigation

Cookies Notification

We use cookies on this site to enhance your user experience. By continuing to browse the site, you consent to the use of our cookies. Learn More
×

System Upgrade on Tue, May 28th, 2024 at 2am (EDT)

Existing users will be able to log into the site and access content. However, E-commerce and registration of new users may not be available for up to 12 hours.
For online purchase, please visit us again. Contact us at [email protected] for any enquiries.

Measured with Sperm Chromatin Dispersion (SCD) Technique: Will DNA Integrity in Spermatozoa Increase After Micronutrient Supplementation? Comparative Controlled Study

    https://doi.org/10.1142/S2661318219500142Cited by:0 (Source: Crossref)

    Background: An elevated DNA Fragmentation Index (DFI) has been associated with male infertility; therefore measuring the sperm nuclear DNA integrity appears to be useful in predicting the ability of spermatozoa to fertilize oocytes.

    Aim: To evaluate the effect of micronutrient supplementation over DFI values in subfertile men.

    Methods: This was a retrospective/comparative study that included a total of 306 subfertile males consulting the clinic from March 2011 to November 2017, who had performed the Sperm Chromatin Dispersion test (SCD) — a method used to detect DNA fragmentation — along with the initial semen analysis. Of the included population, n= 146 had received two daily oral capsules of a standardized combined micronutrient compound (Profertil®, Lenus Pharma, Vienna, Austria) for three months plus lifestyle change (study group). Each capsule contains L-carnitine, L-arginine, coenzyme Q10, zinc, vitamin E, folic acid, glutathione and selenium. Those who did not receive the active treatment (n= 160) but only engaged with lifestyle changes were considered controls. The SCD test was performed in both groups at baseline and after 3 months.

    Results: For the first statistical analysis, patients with an initial mean DFI of >15% were considered (n= 66 [37 were study and 29 were controls]). After 3 months, both groups displayed a significant decrease of mean DFI values. However, this decrease was more evident in the study group as compared to controls (10.54% vs. 14.48%, α= 0.05; p= 0.013). For the second statistical approach the entire population was considered (n= 306). After 3 months, only the study group displayed a significant decrease of the mean initial DFI value (10.16% to 6.49%, p < 0.0001); decrease that was more evident as compared to controls (6.49% vs. 8.82%, α= 0.05; p= 0.000020).

    Conclusions: Among subfertile men with a DFI >15%, both regimes significantly decreased sperm DNA fragmentation; however, when any baseline DFI value was considered, only treatment with the active standardized micronutrient compound achieved a significant better result.

    References

    • Absalan F, Ghannadi A, Kazerooni M, Parifar R, Jamalzadeh F, Amiri S . Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion. J Assist Reprod Genet. 2012; 29(1) :11–4. CrossrefGoogle Scholar
    • Agarwal A, Allamaneni SS . Sperm DNA damage assessment: a test whose time has come. Fertil Steril. 2005; 84(4) :850–3. CrossrefGoogle Scholar
    • Aliabadi E, Soleimani Mehranjani M, Borzoei Z, Talaei-Khozani T, Mirkhani H, Tabesh H . Effects of L-carnitine and L-acetyl-carnitine on testicular sperm motility and chromatin quality. Iran J Reprod Med. 2012; 10(2) :77–82. Google Scholar
    • Alonge S, Melandri M, Leoci R, Lacalandra GM, Caira M, Aiudi GG . The effect of dietary supplementation of vitamin E, selenium, zinc, folic acid, and N-3 polyunsaturated fatty acids on sperm motility and membrane properties in dogs. Animals (Basel). 2019; 9(2): pii:E34. CrossrefGoogle Scholar
    • Alvares TS, Conte-Junior CA, Silva JT, Paschoalin VM . Acute L-Arginine supplementation does not increase nitric oxide production in healthy subjects. Nutr Metab (Lond). 2012; 9(1) :54. CrossrefGoogle Scholar
    • Atig F, Raffa M, Habib BA, Kerkeni A, Saad A, Ajina M . Impact of seminal trace element and glutathione levels on semen quality of Tunisian infertile men. BMC Urol. 2012; 12 :6. CrossrefGoogle Scholar
    • Baker HW . Management of male infertility. Baillieres Best Pract Res Clin Endocrinol Metab. 2000; 14 :409–22. CrossrefGoogle Scholar
    • Carrell DT, Liu L, Peterson CM, Jones KP, Hatasaka HH, Erickson L, Campbell B . Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss. Arch Androl. 2003; 49(1) :49–55. CrossrefGoogle Scholar
    • Chen CH, Lee SS, Chen DC, Chien HH, Chen IC, Chu YN, Liu JY, Chen WH, Wu GJ . Apoptosis and kinematics of ejaculated spermatozoa in patients with varicocele. J Androl. 2004; 25(3) :348–53. CrossrefGoogle Scholar
    • Cheng JB, Zhu J, Ni F, Jiang H . L-carnitine combined with coenzyme Q10 for idiopathic oligoasthenozoospermia: a double-blind randomizided controlled trial. Zhonghua Nan Ke Xue. 2018; 24(1) :33–8. Google Scholar
    • Ebisch IM, Thomas CM, Peters WH, Braat DD, Steegers-Theunissen RP . The importance of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility. Hum Reprod Update. 2007; 13(2) :163–74. CrossrefGoogle Scholar
    • Evenson DP . The Sperm Chromatin Structure Assay (SCSA®) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility. Anim Reprod Sci. 2016; 169 :56–75. CrossrefGoogle Scholar
    • Fallah A, Mohammad-Hasani A, Colagar AH . Zinc is an essential element for male fertility: a review of Zn roles in men’s health, germination, sperm quality, and fertilization. J Reprod Infertil. 2018; 19(2) :69–81. Google Scholar
    • Fernández JL, Muriel L, Rivero MT, Goyanes V, Vazquez R, Alvarez JG . The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl. 2003; 24(1) :59–66. CrossrefGoogle Scholar
    • González-Marin C, Gosálvez J, Roy R . Types, causes detection and repair of DNA fragmentation in animal and human sperm cells. Int J Mol Sci. 2012; 13(11) :14026–52. CrossrefGoogle Scholar
    • Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z . Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res. 1993; 207(1) :202–5. CrossrefGoogle Scholar
    • Hawkes WC, Turek PJ . Effects of dietary selenium on sperm motility in healthy men. J Androl. 2001; 22(5) :764–72. CrossrefGoogle Scholar
    • Isidori AM, Pozza C, Gianfrilli D, Isidori A . Medical treatment to improve sperm quality. Reprod Biomed Online. 2006; 12(6) :704–14. CrossrefGoogle Scholar
    • Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, Zghal K, Fki H, Damak J, Bahloul A . Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl. 2003; 49(2) :83–94. CrossrefGoogle Scholar
    • Lafuente R, González-Comadrán M, Solà I, López G, Brassesco M, Carreras R, Checa MA . Coenzyme Q10 and male infertility: a meta-analysis. J Assist Reprod Genet. 2013; 30(9) :1147–56. CrossrefGoogle Scholar
    • Lewin A, Lavon H . The effect of coenzyme Q10 on sperm motility and function. Mol Aspects Med. 1997; 18 Suppl :S213–9. CrossrefGoogle Scholar
    • Lipovac M, Bodner F, Schütz A, Kurz H, Riedel C, Mair J, Imhof M . Increased Hyaluronan acid binding ability of spermatozoa indicating a better maturity, morphology and higher DNA integrity after micronutrient supplementation. EMJ Urol. 2014; 1 :60–5. CrossrefGoogle Scholar
    • Martínez-Pastor F, Fernández-Santos MR, Domínguez-Rebolledo AE, Esteso MC, Garde JJ . DNA status on thawed semen from fighting bull: a comparison between the SCD and the SCSA tests. Reprod Domest Anim. 2009; 44(3) :424–31. CrossrefGoogle Scholar
    • Ortiz I, Urbano M, Dorado J, Morrell JM, Al-Essawe E, Johannisson A, Hidalgo M . Comparison of DNA fragmentation of frozen-thawed epididymal sperm of dogs using Sperm Chromatin Structure Analysis and Sperm Chromatin Dispersion test. Anim Reprod Sci. 2017; 187 :74–8. CrossrefGoogle Scholar
    • Park YS, Lee SH, Choi HW, Lee HS, Lee JS, Seo JT . Abnormal human sperm parameters contribute to sperm DNA fragmentation in men with varicocele. World J Mens Health. 2018; 36(3) :239–47. CrossrefGoogle Scholar
    • Scott R, MacPherson A, Yates RW, Hussain B, Dixon J . The effect of oral selenium supplementation on human sperm motility. Br J Urol. 1998; 82(1) :76–80. CrossrefGoogle Scholar
    • Showell MG, Mackenzie-Proctor R, Brown J, Yazdani A, Stankiewicz MT, Hart RJ . Antioxidants for male subfertility. Cochrane Database Syst Rev. 2011;(1) :CD007411. Google Scholar
    • Speyer BE, Pizzey AR, Ranieri M, Joshi R, Delhanty JD, Serhal P . Fall in implantation rates following ICSI with sperm with high DNA fragmentation. Hum Reprod. 2010; 25(7) :1609–18. CrossrefGoogle Scholar
    • Swane BG, Kawata A, Behan NA, Williams A, Wade MG, Macfarlane AJ, Yauk CL . Investigating the effects of dietary folic acid on sperm count, DNA damage and mutation in Balb/c mice. Mutat Res. 2012; 737(1–2) :1–7. CrossrefGoogle Scholar
    • Tandara M, Bajić A, Tandara L, Bilić-Zulle L, Šunj M, Kozina V, Goluža T, Jukić M . Sperm DNA integrity testing: big halo is a good predictor of embryo quality and pregnancy after conventional IVF. Andrology. 2014; 2(5) :678–86. CrossrefGoogle Scholar
    • Templeton A . Infertility — epidemiology, aetiology and effective management. Health Bull. 1995; 53 :294–8. Google Scholar
    • Venkatesh S, Singh A, Shamsi MB, Thilagavathi J, Kumar R, Mitra DK, Dada R . Clinical significance of sperm DNA damage threshold value in the assessment of male infertility. Reprod Sci. 2011; 18(10) :1005–13. CrossrefGoogle Scholar
    • Wong WY, Thomas CM, Merkus JM, Zielhuis GA, Steegers-Theunissen RP . Male factor subfertility: possible causes and the impact of nutritional factors. Fertil Steril. 2000; 73(3) :435–42. CrossrefGoogle Scholar
    • Zhang H, Zheng RL . Possible role of nitric oxide on fertile and asthenozoospermic infertile human sperm functions. Free Radic Res. 1996; 25(4) :347–54. CrossrefGoogle Scholar
    • Zhang LH, Qiu Y, Wang KH, Wang Q, Tao G, Wang LG . Measurement of sperm DNA fragmentation using bright-field microscopy: comparison between sperm chromatin dispersion test and terminal uridine nick-end labeling assay. Fertil Steril. 2009; 94(3) :1027–32. CrossrefGoogle Scholar
    • Zhao J, Dong X, Hu X, Long Z, Wang L, Liu Q, Sun B, Wang Q, Wu Q, Li L . Zinc levels in seminal plasma and their correlation with male infertility: a systematic review and meta-analysis. Sci Rep. 2016; 6 :22386. CrossrefGoogle Scholar
    • Zini A, Bielecki R, Phang D, Zenzes MT . Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril. 2001a; 75(4) :674–7. CrossrefGoogle Scholar
    • Zini A, Kamal K, Phang D, Willis J, Jarvi K . Biologic variability of sperm DNA denaturation in infertile men. Urology. 2001b; 58(2) :258–61. CrossrefGoogle Scholar