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Anemone altaica Induces Apoptosis in Human Osteosarcoma Cells

I-Chang Chang

School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan

Department of Orthopedic Surgery, Chung Shan Medical University, Taichung 402, Taiwan

The author contributed equally to this work.

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Tsay-I Chiang

Department of Nursing, College of Medicine & Nursing, Hungkuang University, Taichung 433, Taiwan

The author contributed equally to this work.

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Chun Lo

Graduate Institute of Cancer Biology, China Medical University, Taichung 40402, Taiwan

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Yi-Hua Lai

Department of Biotechnology, Asia University, Taichung 413, Taiwan

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Chia-Herng Yue

Department of Surgery, Tungs' Taichung MetroHarbor Hospital, Taichung 435, Taiwan

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Jer-Yuh Liu

Graduate Institute of Cancer Biology, China Medical University, Taichung 40402, Taiwan

Center for Molecular Medicine, China Medical University Hospital, Taichung 40402, Taiwan

Correspondence to: Dr. Jer-Yuh Liu, Graduate Institute of Cancer Biology, College of Medical, China Medical University, No. 6, Hsueh-Shih Road, Taichung 40402, Taiwan. Tel: (+886) 4-2205-2121 (ext. 7932), Fax: (+886) 4-2234-7028.

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Li-Sung Hsu

Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 402, Taiwan

Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 40201, Taiwan

Correspondence to: Dr. Li-Sung Hsu, Institute of Biochemistry and Biotechnology, Chung Shan Medical University, No. 110, Sec. 1, Jian-Guo N. Road, Taichung 40201, Taiwan. Tel: (+886) 4-2473-0022 (ext. 11682), Fax: (+886) 4-2324-8195.

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and

Chia-Jen Lee

Center for Molecular Medicine, China Medical University Hospital, Taichung 40402, Taiwan

Correspondence to: Dr. Chia-Jen Lee, Center for Molecular Medicine, China Medical University Hospital, No. 6, Hsueh-Shih Road, Taichung 40402, Taiwan. Tel: (+886) 4-2205-2121 (ext. 7917).

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https://doi.org/10.1142/S0192415X15500597Cited by:7

Abstract

In the past decade, no significant improvement has been made in chemotherapy for osteosarcoma (OS). To develop improved agents against OS, we screened 70 species of medicinal plants and treated two human OS cell lines with different agent concentrations. We then examined cell viability using the MTT assay. Results showed that a candidate plant, particularly the rhizomes of Anemone altaica Fisch. ex C. A. Mey aqueous extract (AAE), suppressed the viability of HOS and U2OS cells in a concentration-dependent manner. Flow cytometry analysis revealed that AAE significantly increased the amount of cell shrinkage (Sub-G1 fragments) in HOS and U2OS cells. Moreover, AAE increased cytosolic cytochrome c and Bax, but decreased Bcl-2. The amount of cleaved caspase-3 and poly-(ADP-ribose) polymerase-1 (PARP-1) were significantly increased. AAE suppressed the growth of HOS and U2OS through the intrinsic apoptotic pathway. Data suggest that AAE is cytotoxic to HOS and U2OS cells and has no significant influence on human osteoblast hFOB cells. The high mRNA levels of apoptosis-related factors (PPP1R15A, SQSTM1, HSPA1B, and DDIT4) and cellular proliferation markers (SKA2 and BUB1B) were significantly altered by the AAE treatment of HOS and U2OS cells. Results show that the anticancer activity of AAE could up-regulate the expression of a cluster of genes, especially those in the apoptosis-related factor family and caspase family. Thus, AAE has great potential as a useful therapeutic drug for human OS.

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Published: 29 July 2015

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Vol. 43, No. 05

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Anemone altaica Induces Apoptosis in Human Osteosarcoma Cells

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References

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