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Ocimum gratissimum is Effective in Prevention against Liver Fibrosis in Vivo and in Vitro

Emergency Department and Center of Hyperbaric Oxygen Therapy, Tungs' Taichung MetroHarbor Hospital, Wuchi Dist., Taichung 43503, Taiwan

Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan

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Pei-Yu Chao

Department of Leisure Industry Management, National Chin-Yi University of Technology, Taiping City, Taichung County 41111, Taiwan

Graduate Institute of Basic Medical Science, China Medical University Taichung 40402, Taiwan

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Chin-Chiu Tsai

Department of Physiology, School of Medicine, Chung-Shan Medical University, Taichung 40201, Taiwan

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Hui-Ling Chiou

College of Medical Science and Technology, Chung-Shan Medical University, Taichung 40201, Taiwan

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Yuh-Chyan Liu

Institute of Biochemistry and Biotechnology, Chung-Shan Medical University, Taichung 40201, Taiwan

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Chuan-Chen Hung

Institute of Biochemistry and Biotechnology, Chung-Shan Medical University, Taichung 40201, Taiwan

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Hung-Che Shih

Department of Pharmacology, School of Medicine, College of Medical, Chung-Shan Medical University, Taichung 40201, Taiwan

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Te-Jen Lai

Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan

Department of Psychiatry, Chung Shan Medical University Hospital, Taichung 40201, Taiwan

Correspondence to: Prof. Te-Jen Lai, Institute of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung City 40201, Taiwan. Tel: (+866) 4-2473-0033 (ext. 110010), Fax: (+866) 4-2475-9950.

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and

Jer-Yuh Liu

Graduate Institute of Cancer Biology, China Medical University Taichung 40402, Taiwan

Center for Molecular Medicine, China Medical University Hospital, Taichung 40402, Taiwan

Correspondence to: Prof. Jer-Yuh Liu, Graduate Institute of Cancer Biology, College of Medical, China Medical University, No. 6, Hsueh-Shih Road, Taichung 40402, Taiwan. Tel: (+866) 4-2205-2121 (ext. 7801), Fax: (+866) 4-2234-7028.

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https://doi.org/10.1142/S0192415X14500530Cited by:9

Abstract

Ocimum gratissimum is a traditional herb commonly found in tropical regions, which prevents free radical damage and protects the liver from oxidative stress. In this study, we tested in vivo and in vitro the effectiveness of O. gratissimum extracts (OGEs) in anti-hepatic fibrosis in rats. Male Wistar rats were administered with carbon tetrachloride (CCl₄) by intraperitoneal injection and varying amounts of oral injection of OGE doses (0–40 mg/kg body weight) for 8 weeks. Our experiments showed that OGE significantly reduced liver damage, including steatosis and fibrosis, in a dose-dependent manner, as well as significantly decreased the elevation in plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT). It also inhibited the formation of lipid peroxidative products during CCl₄ treatment. Moreover, OGE-inhibited CCl₄-induced liver collagen accumulation and promoted the expression of catalase, an anti-oxidative enzyme. The inhibition of fibrosis factors α-SMA expression was also observed. In primary cultures, OGE significantly inhibited the serum-induced activation of hepatic stellate cells (HSCs), and the expression of α-SMA and collagen α (I). These data suggest that O. gratissimum possesses anti-hepatic fibrosis properties via its anti-oxidative components.

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Vol. 42, No. 04

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Ocimum gratissimum is Effective in Prevention against Liver Fibrosis in Vivo and in Vitro

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