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(-)-Epigallocatechin-3-Gallate (EGCG) Increases the Viability of Serum-Starved A549 Cells Through Its Effect on Akt

Myung Jin Kim

Department of Oral Pathology, Pusan National University, Yang San, South Korea

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Hyung Il Kim

Department of Dental Materials, Pusan National University, Yang San, South Korea

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Jin Chung

Department of Oral Microbiology, Pusan National University, Yang San, South Korea

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Tae Sung Jeong

Department of Pediatric Dentistry, School of Dentistry, Pusan National University, Yang San, South Korea

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and

Hae Ryoun Park

Department of Oral Pathology, Pusan National University, Yang San, South Korea

Correspondence to: Dr. Hae Ryoun Park, Mool Keum, Beomoe Ri, Room 615, Department of Oral Pathology, School of Dentistry, Pusan National University, Yang San, 626-770, South Korea. Tel: (+82) 51-510-8250, Fax: (+82) 51-510-8249.

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https://doi.org/10.1142/S0192415X09007193Cited by:11

Abstract

The effect of epigallocatechin gallate (EGCG) on cell survival was studied by using serum-starved A549 non-small cell lung carcinoma (NSCLC) cells. A MTT assay showed that EGCG significantly increased the viability of serum-starved A549 cells compared to the control cells, though EGCG at high concentration (~300 μM) had no protective effect against serum withdrawal-induced cell apoptosis. Western blots showed increased immunoreactivity for phospho-Akt and phospho-GSK3β in EGCG-treated cells. To determine the mechanism for Akt phosphorylation, cells were pretreated with various kinase inhibitors before exposure to EGCG. Only LY294002 inhibited Akt activation induced by EGCG, implying that EGCG-induced Akt activation is PI3K dependent. Both phospho-Raf-1 and Raf-1 proteins were significantly decreased, whereas B-raf expression was not altered. This suggests that the Raf kinases have no role in the increased cell survival caused by EGCG. This study has shown that EGCG protects A549 cells from apoptosis induced by serum deprivation via Akt activation and this protective effect may limit the clinical use of EGCG in treating and preventing NSCLC.

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Vol. 37, No. 04

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(-)-Epigallocatechin-3-Gallate (EGCG) Increases the Viability of Serum-Starved A549 Cells Through Its Effect on Akt

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