In vitro Antioxidant, Antimicrobial and Anti-Herpes Simplex Virus Type 1 Activity of Phellodendron amurense Rupr. from China
Abstract
Phellodendron amurense Rupr. bark extracts were examined for antioxidant activity, antimicrobial activity and antiviral activity on herpes simplex virus type 1 (HSV-1). Ethanol extract showed higher content of both total phenolic and flavonoid than aqueous extract. In DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay, the concentration providing 50% inhibition (IC50) values were 6.73 ± 0.87 mg/ml and 4.26 ± 0.59 mg/ml for aqueous and ethanol extracts respectively. Ethanol extract had a much higher antimicrobial activity than aqueous extract. Furthermore, the antiviral activity of the ethanol extract on HSV-1 was also tested. The maximum noncytotoxic concentration was 44.12 μg/ml for RC-37 cells. Plaque formation was inhibited by 74±6% when HSV-1 was pretreated with the extract prior to adsorption, whereas pretreatment of the cells with the extract, added during adsorption or after the adsorption only exhibited above 10% antiviral effect. This study has to some extent validated the medicinal potential of P. amurense bark.
References
- Comp. Immunol. Micro. Infect. Dis. 30, 71 (2007). Medline, ISI, Google Scholar
- Food Chem. 84, 551 (2004), DOI: 10.1016/S0308-8146(03)00278-4. Crossref, ISI, Google Scholar
- LWT 39, 80 (2006), DOI: 10.1016/j.lwt.2004.11.007. Crossref, ISI, Google Scholar
- Antiviral Res. 75, 30 (2007), DOI: 10.1016/j.antiviral.2006.11.006. Crossref, Medline, ISI, Google Scholar
- J. Ethnopharmaco. 91, 105 (2004), DOI: 10.1016/j.jep.2003.12.012. Crossref, Medline, ISI, Google Scholar
- Carbohy. Res. 101, 295 (1982), DOI: 10.1016/S0008-6215(00)81010-1. Crossref, ISI, Google Scholar
- Phytochemistry 37, 19 (1994), DOI: 10.1016/0031-9422(94)85005-4. Crossref, ISI, Google Scholar
- Phytochemistry 48, 285 (1998), DOI: 10.1016/S0031-9422(97)01130-8. Crossref, ISI, Google Scholar
- Phytochemistry 31, 1335 (1992), DOI: 10.1016/0031-9422(92)80285-M. Crossref, ISI, Google Scholar
- Peptides 27, 2069 (2006), DOI: 10.1016/j.peptides.2006.03.004. Crossref, Medline, ISI, Google Scholar
- J. Pharm. Bio. Anal. 40, 173 (2006), DOI: 10.1016/j.jpba.2005.06.017. Crossref, Medline, ISI, Google Scholar
- Flavour Frag. J. 19, 549 (2004), DOI: 10.1002/ffj.1349. Crossref, ISI, Google Scholar
- Life Sci. 75, 2621 (2004), DOI: 10.1016/j.lfs.2004.03.036. Crossref, Medline, ISI, Google Scholar
- Anal. Chim. Acta 78, 495 (1975), DOI: 10.1016/S0003-2670(00)00176-8. Crossref, ISI, Google Scholar
- Phytomedicine 10, 504 (2003), DOI: 10.1078/094471103322331467. Crossref, Medline, ISI, Google Scholar
- Food Chem. 108, 1019 (2008), DOI: 10.1016/j.foodchem.2007.11.046. Crossref, Medline, ISI, Google Scholar
- J. Agric. Food Chem. 42 (1994). Google Scholar
- Phytochemisty 35, 209 (1994). ISI, Google Scholar
| Remember to check out the Most Cited Articles! |
|---|
|
Check out our Chinese Medicine Titles today. |
