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A Purified Extract from Clematis mandshurica Prevents Adenoviral-TRAIL Induced Apoptosis on Rat Articular Chondrocytes

Sung Won Lee

Department of Rheumatology, Dong-A University College of Medicine and Institute of Medical Science, Busan, South Korea

The first two authors contributed equally to this work.

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Hye Jeong Lee

Department of Pharmacology, Dong-A University College of Medicine and Institute of Medical Science, Busan, South Korea

The first two authors contributed equally to this work.

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Jeong Bon Moon

Department of Oral Anatomy and Cell Biology, Pusan National University College of Dentistry, Busan, South Korea

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Sun Mee Choi

Department of Rheumatology, Dong-A University College of Medicine and Institute of Medical Science, Busan, South Korea

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Duk Kyu Kim

Department of Internal Medicine, Dong-A University College of Medicine and Institute of Medical Science, Busan, South Korea

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In Ryoung Kim

Department of Biology, Pusan National University College of Natural Science, Busan, South Korea

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Won Chul Choi

Department of Biology, Pusan National University College of Natural Science, Busan, South Korea

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and

Bong Soo Park

Department of Oral Anatomy and Cell Biology, Pusan National University College of Dentistry, Busan, South Korea

Correspondence to: Dr. Bong Soo Park, Department of Oral Anatomy and Cell Biology, College of Dentistry, Pusan National University, 1-10 Ami-Dong, Seo-Gu, Busan, South Korea 602-739. Tel: (+82) 51-240-7810, Fax: (+82) 51-244-7399.

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https://doi.org/10.1142/S0192415X08005850Cited by:3

Abstract

Since TNF-related apoptosis inducing ligand (TRAIL) is one of several apoptotic stimuli on articular chondrocytes, the modulation of the mechanism mediated by TRAIL could be considered as a novel strategy for the treatment of osteoarthritis (OA). Previous studies demonstrated that Clematis mandshurica prevents staurosporin-induced apoptosis in articular chondrocytes. This study was undertaken to examine whether Clematis mandshurica could prevent TRAIL-induced apoptosis in articular chondrocytes. Our data show that Clematis mandshurica prevents adenoviral TRAIL (Ad-TRAIL)-induced apoptosis in primary cultured articular chondrocytes. Clematis mandshurica prevents Ad-TRAIL-induced down-regulation of 14-3-3 and phosphorylated Akt. In addition, Clematis mandshurica treatment prevents the Ad-TRAIL-induced reduction of the interactions between 14-3-3 with phospho-ser112-Bad and phospho-ser136-Bad, and BcL-xL with phospho-ser155-Bad. A better understanding of the mechanism underlying inhibition of apoptosis in OA chondrocytes by Clematis mandshurica might lead to the development of a new therapeutic strategy for OA.

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A Purified Extract from Clematis mandshurica Prevents Adenoviral-TRAIL Induced Apoptosis on Rat Articular Chondrocytes

Abstract

References

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