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PURIFICATION OF HEAVY CHAIN FERRITIN(FH) FROM RECOMBINANT E. COLI.

    Abstract:

    Iron is an essential nutrient for most organisms, which supplied to them in a protein-iron complex known as ferritin, which is a multimeric protein found in prokaryotes, plants and animals. Expression of ferritin in recombinant E. coli at 37°C led to the accumulation of recombinant ferritin. Insoluble form of ferritin was separated from disrupted cells, followed by various primary separation steps with two kinds of buffers. Collected samples from the steps were purified by DEAE-cellulose gels packed in a column. The fractions from the DEAE column were assayed to gain the amount and the purity of ferritin by using RP-, GF-HPLC.